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KRISP has invested in two high-throughput qPCR equipment (QuantStudio 7 Flex Real Time PCR Systems. We have validated qPCR TaqMan Array Card for many common pathogens and pathogens including panels for Gastrointestinal Panel (38 pathogens), Sexually Transmitted Infections (STI) Panel (12 pathogens) and Respiratory Diagnostic Panel (34 pathogens).

The Applied Biosystems QuantStudio 7 Flex Real-Time PCR System delivers the proven reliability, sensitivity, and accuracy of the Applied Biosystem 7 System in a new industrial design. The QuantStudio 7 Flex System has been optimized to enable the broadest range of quantitative PCR applications, with additional dyes, formats, and automation options. Analysis of hundreds of 384-well plates can be achieved in just minutes.

Some of the applications:

- qPCR

- Absolute quantification

- Gene expression

- Genotyping

- High Resolution Melt (HRM)

- Presence/Absence pf SNPs

We have validated qPCR TaqMan Array Card for many common pathogens and pathogens including panels for:

Gastrointestinal Panel (38 pathogens), including among others: Campylobacter, Enterovirus, Listeria monocytogenes, Norovirus, Rotavirus A and B, Salmonella spp, Staphylococcus aureus, Vibrio cholerae, etc.

Sexually Transmitted Infections (STI) Panel (12 pathogens), including: Herpes simplex virus 1 and 2, Trichomonas vaginalis, Mycoplasma genitalium, Gardnerella vaginalis, Neisseria gonorrhoeae, Chlamydia trachomatis, Lactobacillus crispatus, Prevotella bivia, etc.

Respiratory Diagnostic Panel (34 pathogens), including: Adenovirus, Enterovirus, Influenza (A and B), Rhinovirus, Measles, Mumps, Mycoplasma pneumoniae, Staphylococcus aureus, etc.

Please contact us or use the online request form for TaqMan Array services.

http://www.krisp.org.za/services.php?subcategory=Pathogen%20Identification%20and%20Quantification