Plasma, EDTA whole blood
10 working days
HIV-1 drug resistance testing involves sequencing of the protease (PR) and reverse transcriptase (RT). PR + RT is used normally for protease inhibitor and NRTI and/or NNRTI inhibitor.
Sample requirements and general information:
1. Sample types:
- Fresh plasma collected in a PPT tube / frozen plasma (at least 500 ul)
- EDTA whole blood (at least 3 ml)
2. Sample rejection criteria:
- Haemolysed, lipaemic samples
- Samples without any details / incomplete request forms
- Samples with insufficient volume
- Fresh samples >72 hours from collection
3. Request Form:
• The HIV-1 Drug Resistance test request form (KF87) can be downloaded here (Request Form) and sent together with the samples.
4. Sample transport:
- Fresh samples should be received on ice (2 – 8 °C in a cooler box)
- Frozen samples should be received on dry ice / frozen
5. Opening hours:
- Monday – Friday (8:00 to 16:30)
6. Service details:
7. Factors known to significantly affect the test performance:
- Samples that have undergone >1 freeze / thaw cycle
- Samples with viral loads <1000 RNA copies / ml
8. Clinical advice and report:
- Clinical advice on HIV-1 Drug Resistance test result (mutation levels, mutation scores and interpretation) is available
9. Customer survey and complaints:
- Please complete our customer satisfaction survey here (https://www.surveymonkey.com/r/WQQD3CY)
- Compliments / complaints to the laboratory manager or laboratory director by clicking here.
Diagnostics and Precision Medicine
Drug Resistance Testing
KRISP has been created by the coordinated effort of the University of KwaZulu-Natal (UKZN), the Technology Innovation Agency (TIA) and the South African Medical Research Countil (SAMRC).