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HIV Drug Resistance (Sanger)
Sample type:
Plasma, EDTA whole blood
Turn-around
10 working days
Service details
HIV-1 drug resistance testing involves sequencing of the protease (PR) and reverse transcriptase (RT). PR + RT is used normally for protease inhibitor and NRTI and/or NNRTI inhibitor.
Sample requirements and general information:
1. Sample types:
- Fresh plasma collected in a PPT tube / frozen plasma (at least 500 ul)
- EDTA whole blood (at least 3 ml)
2. Sample rejection criteria:
- Haemolysed, lipaemic samples
- Samples without any details / incomplete request forms
- Samples with insufficient volume
- Fresh samples >72 hours from collection
3. Request Form:
• The HIV-1 Drug Resistance test request form (KF87) can be downloaded here (Request Form) and sent together with the samples.
4. Sample transport:
- Fresh samples should be received on ice (2 – 8 °C in a cooler box)
- Frozen samples should be received on dry ice / frozen
5. Opening hours:
- Monday – Friday (8:00 to 16:30)
6. Service details:
See hardcopy
7. Factors known to significantly affect the test performance:
- Samples that have undergone >1 freeze / thaw cycle
- Samples with viral loads <1000 RNA copies / ml
8. Clinical advice and report:
- Clinical advice on HIV-1 Drug Resistance test result (mutation levels, mutation scores and interpretation) is available
9. Customer survey and complaints:
- Please complete our customer satisfaction survey here (https://www.surveymonkey.com/r/WQQD3CY)
- Compliments / complaints to the laboratory manager or laboratory director by clicking here.
Category
Diagnostics
Sub-category
HIV-1 Drug Resistance Testing by Sanger Sequencing