HIV Drug Resistance (Sanger)

Sample type:

Plasma, EDTA whole blood

Turn-around

10 working days

Service details

HIV-1 drug resistance testing involves sequencing of the protease (PR) and reverse transcriptase (RT). PR + RT is used normally for protease inhibitor and NRTI and/or NNRTI inhibitor.

Sample requirements and general information:

1. Sample types:

- Fresh plasma collected in a PPT tube / frozen plasma (at least 500 ul)

- EDTA whole blood (at least 3 ml)

2. Sample rejection criteria:

- Haemolysed, lipaemic samples

- Samples without any details / incomplete request forms

- Samples with insufficient volume

- Fresh samples >72 hours from collection

3. Request Form:

• The HIV-1 Drug Resistance test request form (KF87) can be downloaded here (Request Form) and sent together with the samples.

4. Sample transport:

- Fresh samples should be received on ice (2 – 8 °C in a cooler box)

- Frozen samples should be received on dry ice / frozen

5. Opening hours:

- Monday – Friday (8:00 to 16:30)

6. Service details:

See hardcopy

7. Factors known to significantly affect the test performance:

- Samples that have undergone >1 freeze / thaw cycle

- Samples with viral loads <1000 RNA copies / ml

8. Clinical advice and report:

- Clinical advice on HIV-1 Drug Resistance test result (mutation levels, mutation scores and interpretation) is available

9. Customer survey and complaints:

- Please complete our customer satisfaction survey here (https://www.surveymonkey.com/r/WQQD3CY)

- Compliments / complaints to the laboratory manager or laboratory director by clicking here.

Category

Diagnostics and Precision Medicine

Sub-category

Drug Resistance Testing

- Avaliable Equipment



Sanger Sequencing: Amplicon, ecology, conservation, agriculture, medical, HIV drug resistance, HLA typing

KRISP has been created by the coordinated effort of the University of KwaZulu-Natal (UKZN), the Technology Innovation Agency (TIA) and the South African Medical Research Countil (SAMRC).


Location: K-RITH Tower Building
Nelson R Mandela School of Medicine, UKZN
719 Umbilo Road, Durban, South Africa.
Director: Prof. Tulio de Oliveira