Re-emergence of DENV-3 in Paraguay After Two Decades: A Genomic and Epidemiological Investigation

Publication

Title: Re-emergence of DENV-3 in Paraguay After Two Decades: A Genomic and Epidemiological Investigation
Authors: Cantero C, Vazquez C, Gonzalez S, Rojas A, Fleitas F, Barrios J, Gomez de la Fuente A, Villalba S, Torales M, Ijeda A, Fonseca V, Junior Alcantara L, Giovanetti M.
Journal: Emerging Microbes & Infections,: (2026)

Abstract

We report the re-emergence of dengue virus serotype 3 (DENV-3) in Paraguay after 15 years of absence. Twelve laboratory-confirmed cases were detected in early 2025 in the Asunción metropolitan region. Genomic sequencing identified DENV-3 genotype III, lineage B.3.2, indicating a new introduction of an emerging lineage rather than renewed local transmission. Phylogenetic analyses support recent introduction events, underscoring the importance of strengthened genomic surveillance to enable early detection, track viral introductions, and inform public health responses in settings with intense dengue transmission.

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Citation: Cantero C, Vazquez C, Gonzalez S, Rojas A, Fleitas F, Barrios J, Gomez de la Fuente A, Villalba S, Torales M, Ijeda A, Fonseca V, Junior Alcantara L, Giovanetti M. Re-emergence of DENV-3 in Paraguay After Two Decades: A Genomic and Epidemiological Investigation Emerging Microbes & Infections,: (2026).